Cat. No. ARC0012
4T1 is a mouse breast cancer cell line, established from a malignant neoplasm of the mouse mammary gland. The 4T1 cells are tumorigenic and highly metastatic. The transplantable tumor can spontaneously metastasize from the primary site to multiple tissues, including the lymph nodes, blood, liver, lung, brain, and bone. This progressive process of tumor growth and metastatic spread of 4T1 cells closely mimics human breast cancer; therefore, this cell line is regarded as an animal model of stage IV human breast cancer for studying breast cancer progression, immune response, and metastasis. <br> <br> Synonym: 4T1-A; 4T1.0; 4T1/WT <br> <br><span style="font-size:14px;">B. A. Pulaski and S. Ostrand‐Rosenberg, “Mouse 4T1 Breast Tumor Model,” <em>CP in Immunology</em>, vol. 39, no. 1, Oct. 2000, doi: 10.1002/0471142735.im2002s39.
| Morphology | Epithelial-like |
| Growth Mode | Adherent |
| Age | Unknown |
| Sex of Donor | Female |
| Derived From Site | In situ; Mammary gland |
| Temperature | 37°C |
| Atmosphere | 5% CO₂ |
| Sterility testing | Daily monitoring confirms that the cells are free from bacterial, yeast, and fungal contamination. |
| Mycoplasma testing | Negative for mycoplasma through PCR analysis |
Intended Use: This product is intended for laboratory in vitro use only. lt is not intended for diagnostic, therapeutic, or clinical applications.
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