Description

The ELDR Knockout U-251MG Cell Line is a CRISPR/Cas9-edited knockout cell line designed for functional studies of the long non-coding RNA ELDR in glioblastoma. This product features targeted disruption of the ELDR gene in the U-251MG human glioblastoma cell line, resulting in loss of ELDR expression. The knockout model serves as a powerful tool for investigating ELDR-dependent molecular mechanisms without off-target interference from transient silencing approaches.

The parental U-251MG cell line is a well-characterized glioblastoma model derived from a male patient, widely employed in neuro-oncology research. These cells retain key genetic and phenotypic characteristics of primary glioblastoma, including aggressive growth and invasive potential. Their established background makes them a reliable platform for dissecting signaling pathways that drive glioblastoma progression and therapeutic resistance.

ELDR is a long non-coding RNA upregulated by EGFR signaling and the transcription factor STAT3. Mechanistically, ELDR acts as a competing endogenous RNA (ceRNA) that sponges tumor-suppressive microRNAs, such as miR-744-5p and miR-31-5p, thereby relieving repression of oncogenic targets. This leads to enhanced expression of downstream effectors including MYC, cyclin D1, MMP2, and MMP9, and activation of the PI3K-AKT and MAPK-ERK pathways. Consequently, ELDR integrates signals from EGFR, RAS, PI3K, AKT, mTOR, and ERK1/2 to promote glioblastoma cell proliferation, migration, and invasion.

Disruption of ELDR in the U-251MG background enables precise dissection of its contributions to glioblastoma pathogenesis. This model is particularly suited for examining how ELDR loss affects EGFR-driven signaling networks, cell cycle progression, and invasive properties. Researchers can directly assess the functional impact of ELDR ablation on downstream targets and pathway activity, providing insights into resistance mechanisms and potential therapeutic vulnerabilities in glioblastoma.

The ELDR Knockout U-251MG Cell Line supports a broad range of applications, including glioblastoma mechanistic studies, drug resistance profiling, and validation of lncRNA-targeted therapies. Standard assays such as RT-qPCR and western blotting confirm ELDR knockout and downstream impact, while MTT/CCK-8, Transwell migration/invasion, and xenograft models enable functional phenotyping. RNA immunoprecipitation (RIP) can be used to explore ELDR-miRNA interactions. For additional information, please contact Ascent Research.