Cat. No. ARG43869
The G3bp1 Knockout AML12 Cell Line is a CRISPR/Cas9-edited knockout cell line derived from AML12 murine hepatocytes, featuring disruption of the G3bp1 gene. G3bp1 is an RNA-binding protein that nucleates stress granules and modulates Ras signaling through interactions with RasGAP, Caprin-1, and USP10, influencing mRNA stability and cell survival. This knockout model is ideal for studying stress granule biology, mRNA regulation, and Ras pathway crosstalk in a hepatocyte background, with key applications in liver cancer, viral infection, and drug resistance research. Representative assays include stress granule immunofluorescence, RNA immunoprecipitation, and cell viability analysis under stress conditions.
| Host Cell | AML12 |
| Gene Name | G3BP1 |
| Gene Identifier | NCBI Gene ID 27041 |
| Storage | Liquid nitrogen (LN2) |
| Temperature | 37°C |
| Atmosphere | 5% CO₂ |
| Sterility testing | The bacterial, yeast, and fungi are not detected in these cells by daily monitor. |
| Mycoplasma testing | Negative for mycoplasma through PCR analysis |
Intended Use: This product is intended for laboratory in vitro use only. lt is not intended for diagnostic, therapeutic, or clinical applications.
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This product is provided "AS IS". For Research Use Only. Not for human or animal therapeutic use.
The G3bp1 Knockout AML12 Cell Line is a CRISPR/Cas9-edited knockout cell line derived from the AML12 murine hepatocyte line, engineered to disrupt the G3bp1 gene. This cell-based knockout model provides a stable loss-of-function system for investigating the roles of G3bp1 in stress granule dynamics, mRNA regulation, and Ras signaling in a hepatocyte context.
AML12 is a non-transformed mouse hepatocyte cell line originally isolated from transgenic mice overexpressing human transforming growth factor alpha (TGF-??). As immortalized liver parenchymal cells, AML12 cells retain key hepatocyte features, including metabolic activity, detoxification capacity, and protein synthesis functions, making them a widely used model for liver biology, hepatocellular carcinoma research, and drug metabolism studies. This background provides a physiologically relevant platform for studying G3bp1 function in hepatic stress responses and disease pathology.
G3bp1 (Ras GTPase-activating protein-binding protein 1) is an RNA-binding protein that serves as a central nucleator of stress granules??cytoplasmic ribonucleoprotein aggregates formed in response to cellular stress. Under stress conditions such as heat shock, oxidative stress, or arsenite exposure, G3bp1 oligomerizes and recruits key components including Caprin-1, USP10, TIA-1, and PABP1, thereby regulating mRNA stability and translation. G3bp1 also interacts directly with RasGAP, modulating Ras signaling cascades and influencing cell proliferation and survival. Its activity is regulated by upstream factors including FAK and PKR-mediated phosphorylation, and it controls downstream targets such as p53 and the NF-??B pathway, integrating stress sensing with cell fate decisions.
In the hepatic context, G3bp1 plays critical roles in liver pathophysiology. Hepatocytes are routinely exposed to metabolic, oxidative, and chemical stressors; dysregulated stress granule formation and impaired mRNA control contribute to liver diseases such as cancer, steatosis, and viral hepatitis. The AML12 G3bp1 knockout model enables dissecting how loss of G3bp1 alters stress responses, Ras-driven proliferative signaling, and innate immune pathways in liver cells. This model is particularly relevant for studying mechanisms of hepatocellular carcinoma, drug resistance, and liver-specific viral-host interactions, where G3bp1 has been implicated in modulating viral replication and cellular antiviral defenses.
Researchers can employ this knockout cell line in a wide range of assays. Immunofluorescence microscopy allows direct visualization of stress granule dynamics and composition; western blotting confirms G3BP1 protein absence and assesses downstream signaling changes; RT-qPCR and RNA immunoprecipitation (RIP) enable analysis of specific mRNA targets and interactions; polysome profiling reveals translational regulation; and cell viability assays under stress conditions evaluate functional consequences. These applications support studies in cancer biology, neurodegeneration, and infectious disease, wherever G3bp1-mediated stress responses and signaling networks are relevant. For further technical specifications and ordering details, please contact Ascent Research.
