Description

The PCDH9 Knockout HEK293T Cell Line is a human knockout cell line generated by CRISPR/Cas9-mediated disruption of the PCDH9 gene, encoding protocadherin-9. This stable loss-of-function model provides an isogenic system for investigating PCDH9-dependent cellular processes in a defined genetic background.

The parental HEK293T cell line is a human embryonic kidney line transformed with SV40 large T antigen, exhibiting adherent epithelial morphology and exceptionally high transfection efficiency. It is widely utilized for protein expression, virus production, and functional genomics studies, ensuring reproducibility across diverse assay platforms.

Protocadherin-9 is a calcium-dependent transmembrane adhesion molecule that mediates homophilic cell-cell contacts. It interacts with catenins, including beta-catenin and p120 catenin, to influence Wnt/beta-catenin signaling and Rho family GTPases such as RAC1 and CDC42. Loss of PCDH9 disrupts these interactions, potentially altering beta-catenin localization, TCF/LEF transcriptional activity, and actin cytoskeletal dynamics driven by Rho GTPases.

In HEK293T cells, the knockout enables dissection of adhesion-dependent signaling in a system expressing core Wnt and MAPK/ERK pathway components. This host background supports rescue experiments, luciferase reporter assays, and co-expression studies, allowing exploration of PCDH9??s role downstream of WNT ligands and Frizzled receptors and its impact on transcription and cytoskeletal organization.

Applications include cell adhesion and migration/invasion assays, co-immunoprecipitation with beta-catenin or NHERF1, TOP/FOP Flash reporter assays for Wnt activity, and actin staining. The line is relevant to cancer biology, neurodevelopmental disorder research, and tumor suppressor studies. For further information, please contact Ascent Research.