Description

The Bsg Knockout RAW 264.7 Cell Line is a CRISPR/Cas9-edited knockout cell line derived from the mouse RAW 264.7 macrophage line. This product offers a stable, loss-of-function model for studying Basigin (CD147) functions in innate immune cells. The gene encoding Basigin has been disrupted via CRISPR/Cas9, leading to ablation of protein expression and eliminating its downstream signaling activities. This engineered line provides researchers with a consistent tool to dissect Basigin-dependent processes without gene silencing artifacts.

RAW 264.7 is a macrophage-like cell line from a BALB/c mouse Abelson virus-induced tumor, widely used for innate immunity studies due to its phagocytic, antigen-presenting, and cytokine-secreting capacities. Its genetic tractability makes it ideal for CRISPR-based knockout, enabling dissection of gene function in inflammatory responses and host-pathogen interactions.

Basigin (CD147) is a transmembrane glycoprotein that induces matrix metalloproteinases, primarily MMP-9 and MMP-1, via ERK1/2 and NF-kB pathways downstream of TNF-alpha, IL-1beta, and TGF-beta. It interacts with cyclophilin A, cyclophilin B, monocarboxylate transporters MCT1 and MCT4, and integrins ??3??1 and ??6??1, linking metabolic regulation, cell adhesion, and immune signaling. These interactions are critical for lymphocyte activation, spermatogenesis, and invasive cellular behaviors.

In RAW 264.7 macrophages, Bsg knockout abrogates Basigin-mediated MMP induction and integrin adhesive functions, impairing matrix degradation, migration, and inflammatory cytokine output. Loss of Basigin likely blunts ERK/NF-kB signaling, attenuates cyclophilin and MCT interactions, and reduces immune synapse formation. This model thus represents a macrophage with compromised pro-inflammatory and tissue-infiltrating capabilities, providing a platform to study macrophage roles in chronic inflammation, cancer, and metabolic diseases.

This knockout line supports diverse assays such as Western blotting, RT-qPCR, flow cytometry, MMP activity measurement, and migration/invasion studies, enabling detailed functional analyses. It serves research in cancer metastasis, rheumatoid arthritis, malaria receptor biology, and CD147-targeted drug discovery. For technical inquiries, please contact Ascent Research.