GOLGA8B Knockout SW620 Cell Line

The GOLGA8B Knockout SW620 Cell Line is a CRISPR/Cas9-edited knockout cell line designed to disrupt the GOLGA8B gene in the human SW620 colorectal adenocarcinoma cell line. This knockout model provides a loss-of-function system for investigating the roles of the GOLGA8B golgin in Golgi architecture, vesicle tethering, and secretory pathway dynamics. By using CRISPR/Cas9-mediated gene disruption, researchers can study the consequences of GOLGA8B ablation on cellular trafficking and cancer-related processes.

The SW620 cell line was established from a metastatic lymph node of a 51-year-old Caucasian male with Dukes’ type C colorectal adenocarcinoma. These cells exhibit a highly metastatic phenotype and are widely used as a model for colon cancer metastasis. The SW620 background offers a relevant context for examining how Golgi-associated proteins contribute to malignant progression, given the line’s established invasive properties and its origin from a secondary tumor site.

GOLGA8B encodes a cis-Golgi golgin protein that functions in tethering transport vesicles to the Golgi membrane, a critical step for maintaining Golgi stack organization and efficient secretory trafficking. Its activity is regulated by ARF GTPases and Rab GTPases such as ARF1 and Rab1, and it interacts with key Golgi structural components including GM130, GOLGA2, and GRASP65. GOLGA8B participates in organizing COPI and COPII coat-mediated trafficking and influences downstream effectors like Golgi reassembly stacking proteins and cytoskeletal filaments. Through these interactions, GOLGA8B modulates vesicle-mediated transport and the secretory pathway, with potential impacts on cell migration and matrix remodeling.

In the context of colorectal cancer, disruption of GOLGA8B may compromise Golgi morphology and secretory efficiency, potentially impairing processes essential for metastasis such as cell migration, invasion, and extracellular matrix degradation. The SW620 cell line, with its inherent metastatic capacity, provides a physiologically relevant model to dissect the contribution of Golgi-dependent secretion to cancer cell dissemination. This knockout cell line enables researchers to assess how loss of GOLGA8B affects key metastatic behaviors and to identify vulnerabilities in the Golgi-dependent trafficking of pro-invasive factors.

The GOLGA8B Knockout SW620 Cell Line is suited for a range of experimental applications, including immunofluorescence and electron microscopy studies of Golgi architecture, western blotting analysis of Golgi protein expression, and functional assays such as cell migration, invasion, and proliferation. Additionally, this model can be employed in RNA-seq experiments to uncover transcriptional changes linked to GOLGA8B loss, and for drug screening efforts aimed at targeting Golgi dynamics in metastatic cancers. Together, these applications facilitate a deeper understanding of golgin biology and the secretory pathway??s role in cancer progression. For additional details or technical support, please contact Ascent Research.