In Stock Cell Lines
Mus musculus (Mouse)
Large intestine (colon)
Adherent
The Tpt1 Knockout CT26.WT Cell Line is a CRISPR/Cas9-edited knockout cell line derived from the murine CT26.WT colorectal carcinoma model. It features targeted disruption of Tpt1, which encodes the translationally-controlled tumor protein implicated in apoptosis regulation via interactions with Mcl-1 and p53. This knockout model is designed for investigating colorectal cancer biology, apoptosis resistance mechanisms, and drug responses. It supports assays such as western blotting, cell viability, and xenograft studies, and is suitable for syngeneic tumor immunology research using BALB/c mice. For further details, contact Ascent Research.
DISC1 Knockout U-251MG Cell Line
Cat. No. ARG43816
ADIRF Knockout 786-O Polyclonal Cells
Cat. No. ARG24832
DOCK1 Knockout HEK293T Polyclonal Cells
Cat. No. ARG39533
MYCBP2 Knockout HEK293T Polyclonal Cells
Cat. No. ARG4638
PGRMC2 Knockout HEK293T Polyclonal Cells
Cat. No. ARG3755
Rabbit Thymic Fibroblast Medium
Cat. No. ARM0767
The Tpt1 Knockout CT26.WT Cell Line is a CRISPR/Cas9-edited knockout cell line derived from Mus musculus CT26.WT colorectal carcinoma cells. This model features targeted disruption of the Tpt1 gene, generating a stable loss-of-function system for studying translationally-controlled tumor protein (TPT1) in cancer biology. It provides a reliable platform for mechanistic and pharmacological investigations.
CT26.WT is a BALB/c mouse colorectal adenocarcinoma line chemically induced by N-nitroso-N-methylurethane. As a syngeneic tumor model, it enables in vivo studies in immunocompetent BALB/c hosts, reflecting key aspects of colorectal cancer progression, tumor microenvironment, and immune interactions. The line??s aggressive tumorigenicity makes it ideal for translational oncology research.
TPT1, encoded by Tpt1, is a multifunctional protein regulating proliferation, apoptosis, and stress responses. It is repressed by p53 and activated by mTORC1 and cellular stress. TPT1 interacts with Mcl-1, p53, Bax, and tubulin, influencing the mTOR and p53 pathways. It stabilizes Mcl-1, inhibiting apoptosis, and modulates Bcl-xL. Disruption of Tpt1 abolishes its anti-apoptotic function, enhancing p53-mediated apoptosis and impairing mTORC1 signaling, thereby sensitizing cells to stress-induced death.
In colorectal carcinoma, Tpt1 knockout disrupts apoptosis resistance, shifting signaling toward pro-apoptotic effectors like Bax and relieving Mcl-1-mediated survival. This sensitizes CT26.WT cells to chemotherapeutics such as 5-fluorouracil and oxaliplatin. Additionally, loss of the histamine-releasing factor TPT1 links the model to allergic inflammation studies, bridging tumor biology and immune regulation.
This cell line supports western blotting for Tpt1, Mcl-1, and p53; RT-qPCR; apoptosis assays (Annexin V/PI); cell viability and colony formation; xenograft tumor growth; and drug sensitivity screens. It is also suited for syngeneic immunology studies evaluating tumor-immune dynamics. For detailed product inquiries, please contact Ascent Research.
