In Stock Cell Lines
Homo sapiens (Human)
Kidney
Adherent
The NR4A1 Knockout HEK293T Cell Line is a CRISPR/Cas9-edited knockout cell line that disrupts the NR4A1 gene in HEK293T human embryonic kidney epithelial cells. NR4A1 is a stress-inducible orphan nuclear receptor and transcription factor that regulates apoptosis via transactivation of BCL2L11 (Bim) and BNIP3L (NIX), and controls metabolic pathways through FBP1 and PEPCK. It integrates signals from MAPK pathways and interacts with cofactors such as p300. Knockout of NR4A1 in HEK293T provides a powerful model to study apoptosis, metabolic regulation, and inflammation. Applications include flow cytometric cell death assays, metabolic flux analysis, luciferase reporter gene assays, and RT-qPCR-based analysis of NR4A1 target genes. Contact Ascent Research for additional details.
GCH1 Knockout HT29 Polyclonal Cells
Cat. No. ARG14530
FITM2 Knockout HT29 Polyclonal Cells
Cat. No. ARG13906
BSDC1 Knockout Hela Polyclonal Cells
Cat. No. ARG23500
ATG14 Knockout HEK293T Polyclonal Cells
Cat. No. ARG25776
ATG7 Knockout SK-HEP-1 Polyclonal Cells
Cat. No. ARG32301
CLK3 Knockout AGS Polyclonal Cells
Cat. No. ARG2223
The NR4A1 Knockout HEK293T Cell Line is a targeted CRISPR/Cas9-edited knockout cell line that disrupts the NR4A1 locus, effectively ablating NR4A1 expression in HEK293T cells. This knockout model enables researchers to dissect NR4A1-specific functions in transcriptional control, apoptosis, and metabolic signaling, free from interference by endogenous NR4A1 activity.
HEK293T cells are a human embryonic kidney epithelial cell line that stably expresses the SV40 large T-antigen, facilitating high levels of transient transfection and protein expression. Their robust growth and extensive characterization make them a favored host for genetic perturbation studies, providing a reproducible platform for investigating gene function and cellular signaling.
NR4A1, an immediate-early orphan nuclear receptor, is rapidly induced by diverse stimuli such as growth factors, cytokines, cAMP, and calcium. Activation involves phosphorylation by ERK1/2, p38, and JNK upstream kinases. NR4A1 acts as a transcription factor, directly promoting expression of pro-apoptotic genes (BCL2L11/Bim, BNIP3L/NIX) and metabolic regulators (FBP1, PEPCK), while modulating inflammatory cytokines. It associates with coactivators p300/SRC-1 and corepressors NCoR/SMRT, and participates in heterodimer formation with RXR and functional interaction with PPAR??. Consequently, NR4A1 integrates signals from MAPK/ERK, PI3K/AKT, and Wnt/??-catenin pathways to effect downstream apoptosis and metabolic responses.
In HEK293T cells, NR4A1 knockout provides a clean background to dissect its involvement in stress-induced apoptosis and metabolic reprogramming. The well-studied MAPK and PI3K/AKT signaling competency of HEK293T cells allows researchers to precisely map NR4A1-dependent versus -independent events upon stimulation. This model is particularly valuable for examining mechanisms linking extracellular signals to mitochondrial apoptosis and for identifying NR4A1 transcriptional targets in a human epithelial context.
Typical applications include apoptosis analysis via flow cytometry coupled with caspase-3/7 activity assays, metabolic flux analysis to monitor changes in glucose utilization and mitochondrial respiration, and ChIP-qPCR or luciferase reporter assays to probe NR4A1 transcriptional activity and promoter occupancy. The knockout cell line is also suitable for investigating steroidogenic enzyme gene regulation, neuroprotective signaling, and cytokine-driven inflammatory responses, utilizing techniques such as RT-qPCR and western blotting. For additional technical details or assistance, please contact Ascent Research.
